Hotspot

psaJ-rpl33

LSC · 64958-65427 · 470 bp

Back to Lespedeza

Pi 0.0097

MarkerSeek score 51.60

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 470
variable_sites: 17
pi: 0.0097
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTGGCGGGTCTCTTGATAGA	CACAACCGGTACACTCCAAA	50.0	50.0	58.1	58.0	597-615 bp	1.0	81.2
psaJ-rpl33_p2	TACTCTATGGTTCGGGGCTT	CACAACCGGTACACTCCAAA	50.0	50.0	57.8	58.0	616-634 bp	1.0	80.8
psaJ-rpl33_p3	CTCTATGGTTCGGGGCTTTG	CACAACCGGTACACTCCAAA	55.0	50.0	58.3	58.0	614-632 bp	1.0	79.6
psaJ-rpl33_p4	AGTACTCTATGGTTCGGGGC	CACAACCGGTACACTCCAAA	55.0	50.0	58.6	58.0	618-636 bp	1.0	77.5
psaJ-rpl33_p5	GTACTCTATGGTTCGGGGCT	CACAACCGGTACACTCCAAA	55.0	50.0	58.6	58.0	617-635 bp	1.0	77.5