Hotspot

trnP-UGG-psaJ

LSC · 64571-64822 · 252 bp

Back to Lespedeza

Pi 0.0176

MarkerSeek score 51.50

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 252
variable_sites: 17
pi: 0.0176
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	CGAACTGAACTAAGAGCGCT	GGTGCCACGGAAAGATATGT	50.0	50.0	58.0	58.0	565-574 bp	1.0	84.8
trnP-UGG-psaJ_p2	CGAACTGAACTAAGAGCGCT	GACCAGATTCCGAGTTCCAC	50.0	55.0	58.0	58.0	842-851 bp	1.0	84.0
trnP-UGG-psaJ_p3	GCGTTCTACCGAACTGAACT	GACCAGATTCCGAGTTCCAC	50.0	55.0	57.9	58.0	851-860 bp	1.0	83.6
trnP-UGG-psaJ_p4	CGAACTGAACTAAGAGCGCT	CACAACCGGTACACTCCAAA	50.0	50.0	58.0	58.0	1199-1217 bp	1.0	83.4
trnP-UGG-psaJ_p5	CGAACTGAACTAAGAGCGCT	TTTACCCTTGGCCATGAACC	50.0	50.0	58.0	58.1	1159-1177 bp	1.0	83.3