Hotspot

petA-psbJ

LSC · 61083-61947 · 865 bp

Back to Lespedeza

Pi 0.0117

MarkerSeek score 55.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 865
variable_sites: 43
pi: 0.0117
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGTCTCTTGTTCT	CATATTCCGGATTGGGCTCA	50.0	50.0	57.2	57.4	1001-1039 bp	1.0	73.0
petA-psbJ_p2	GCGTCCAAGGTCTCTTGTTC	CATATTCCGGATTGGGCTCA	55.0	50.0	58.9	57.4	1002-1040 bp	1.0	72.7
petA-psbJ_p3	TTACGCGTCCAAGGTCTCTT	CATATTCCGGATTGGGCTCA	50.0	50.0	59.0	57.4	1006-1044 bp	1.0	71.2
petA-psbJ_p4	CGTCCAAGGTCTCTTGTTCTT	CATATTCCGGATTGGGCTCA	47.6	50.0	57.9	57.4	1001-1039 bp	1.0	70.6
petA-psbJ_p5	CGTCCAAGGTCTCTTGTTCT	TATTCCGGATTGGGCTCATC	50.0	50.0	57.2	57.1	999-1037 bp	1.0	70.6