Hotspot

ycf4-cemA

LSC · 58795-59231 · 437 bp

Back to Lespedeza

Pi 0.0133

MarkerSeek score 59.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 437
variable_sites: 18
pi: 0.0133
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CCCTTGACTCGCATTGAAGA	ACACCAGGGCAAAAAGACTA	50.0	45.0	57.9	57.0	592-606 bp	1.0	75.2
ycf4-cemA_p2	CCCTTGACTCGCATTGAAGA	TACACCAGGGCAAAAAGACT	50.0	45.0	57.9	57.0	593-607 bp	1.0	75.2
ycf4-cemA_p3	TCCCTTGACTCGCATTGAAG	ACACCAGGGCAAAAAGACTA	50.0	45.0	57.9	57.0	593-607 bp	1.0	75.2
ycf4-cemA_p4	TCCCTTGACTCGCATTGAAG	TACACCAGGGCAAAAAGACT	50.0	45.0	57.9	57.0	594-608 bp	1.0	75.2
ycf4-cemA_p5	AGGTTGTTGGGTGTACCTCT	ACACCAGGGCAAAAAGACTA	50.0	45.0	58.2	57.0	526-540 bp	1.0	75.1