Hotspot

atpA-trnR-UCU

LSC · 50789-51123 · 335 bp

Back to Lespedeza

Pi 0.00951

MarkerSeek score 51.60

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 335
variable_sites: 7
pi: 0.00951
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	CATTCACTGGGGAAGCAGAA	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	422-456 bp	1.0	70.1
atpA-trnR-UCU_p2	AAGACATTCACTGGGGAAGC	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	426-460 bp	1.0	70.1
atpA-trnR-UCU_p3	GGAAGCAGAAGCCCTTTTGA	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.4	57.3	412-446 bp	1.0	68.7
atpA-trnR-UCU_p4	ATTCACTGGGGAAGCAGAAG	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.5	57.3	421-455 bp	1.0	67.7
atpA-trnR-UCU_p5	GACATTCACTGGGGAAGCAG	GGTTCAAATCCTATTGGACGC	55.0	47.6	58.5	57.3	424-458 bp	1.0	67.4