Hotspot

trnC-GCA-rpoB

LSC · 32728-33878 · 1151 bp

Back to Lespedeza

Pi 0.00738

MarkerSeek score 51.00

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1151
variable_sites: 30
pi: 0.00738
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CCGGATTTGAACTGGGGAAA	AAAAGCCCCTCTGTTAAGCC	50.0	50.0	58.1	58.1	1320-1344 bp	1.0	81.0
trnC-GCA-rpoB_p2	AAAAGGATTTGCAGTCCCCC	AAAAGCCCCTCTGTTAAGCC	50.0	50.0	58.4	58.1	1302-1326 bp	1.0	78.9
trnC-GCA-rpoB_p3	CCGGATTTGAACTGGGGAAA	CCCTCTGTTAAGCCCTGATC	50.0	55.0	58.1	57.4	1314-1338 bp	1.0	76.6
trnC-GCA-rpoB_p4	CCGGATTTGAACTGGGGAAA	CCCCTCTGTTAAGCCCTGAT	50.0	55.0	58.1	58.8	1315-1339 bp	1.0	75.4
trnC-GCA-rpoB_p5	GACACCCGGATTTGAACTGG	AAAAGCCCCTCTGTTAAGCC	55.0	50.0	58.8	58.1	1325-1349 bp	1.0	75.0