Hotspot

psbD-trnT-GGU

LSC · 28323-29694 · 1372 bp

Back to Lespedeza

Pi 0.00707
MarkerSeek score 53.20
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
LSC
length_bp
1372
variable_sites
33
pi
0.00707
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 CACAAAAACGAAACGGTCCC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1513-1548 bp 1.0 81.1
psbD-trnT-GGU_p2 CCACAAAAACGAAACGGTCC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1514-1549 bp 1.0 81.1
psbD-trnT-GGU_p3 CCCACAAAAACGAAACGGTC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1515-1550 bp 1.0 81.1
psbD-trnT-GGU_p4 CACAAAAACGAAACGGTCCC TCAGTGGTAGAGTAACGCCA 50.0 50.0 58.2 58.1 1516-1551 bp 1.0 80.9
psbD-trnT-GGU_p5 CCACAAAAACGAAACGGTCC TCAGTGGTAGAGTAACGCCA 50.0 50.0 58.2 58.1 1517-1552 bp 1.0 80.9