Hotspot

ycf1

SSC · 123910-129627 · 5718 bp

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Pi 0.00914

MarkerSeek score 44.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5718
variable_sites: 52
pi: 0.00914
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGAGTCTTTCTTACCTTCACA	TCACAAAACTGGCGAAATAGA	38.1	38.1	54.2	55.8	219 bp	1.0	42.6
ycf1_p2	TGAGTCTTTCTTACCTTCACAA	TCACAAAACTGGCGAAATAGA	36.4	38.1	55.0	55.8	219 bp	1.0	42.6
ycf1_p3	TGAGTCTTTCTTACCTTCACA	TCAATATCACAAAACTGGCGA	38.1	38.1	54.2	55.9	225 bp	1.0	42.5
ycf1_p4	TGAGTCTTTCTTACCTTCACAA	TCAATATCACAAAACTGGCGA	36.4	38.1	55.0	55.9	225 bp	1.0	42.5
ycf1_p5	TGAGTCTTTCTTACCTTCACA	TCAATATCACAAAACTGGCGAA	38.1	36.4	54.2	56.6	225 bp	1.0	42.5