Hotspot

rps8

LSC · 79057-79461 · 405 bp

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Pi 0.0148

MarkerSeek score 52.00

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 6
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	AGGAAGTTCGAATACAATTTGCA	TCAAAATTAGGGAGAAACAAGA	34.8	31.8	57.0	53.3	475 bp	1.0	41.0
rps8_p2	AGGAAGTTCGAATACAATTTGCA	TCAAAATTAGGGAGAAACAAGAA	34.8	30.4	57.0	54.1	475 bp	1.0	41.0
rps8_p3	AGGAAGTTCGAATACAATTTGCA	ATCAAAATTAGGGAGAAACAAGA	34.8	30.4	57.0	53.9	476 bp	1.0	41.0
rps8_p4	GGAAGTTCGAATACAATTTGCA	TCAAAATTAGGGAGAAACAAGA	36.4	31.8	55.6	53.3	474 bp	1.0	41.0
rps8_p5	AGGAAGTTCGAATACAATTTGCAT	TCAAAATTAGGGAGAAACAAGA	33.3	31.8	57.5	53.3	475 bp	1.0	41.0