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psbL-psbF

LSC · 63056-63081 · 26 bp

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Pi 0.0385

MarkerSeek score 62.80

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 1
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	CAACATTTTGTTCGTTCGGGT	TCAGCAATGCAGTTCATCCA	42.9	45.0	57.9	57.8	87 bp	1.0	70.7
psbL-psbF_p2	CCCCCAGTAGAGACTAGTACG	TCAGCAATGCAGTTCATCCA	57.1	45.0	58.2	57.8	116 bp	1.0	69.9
psbL-psbF_p3	CAACATTTTGTTCGTTCGGGT	GCAATGCAGTTCATCCAACG	42.9	50.0	57.9	58.4	84 bp	1.0	69.3
psbL-psbF_p4	TTGTTCGTTCGGGTTTGATTG	TCAGCAATGCAGTTCATCCA	42.9	45.0	57.7	57.8	80 bp	1.0	68.6
psbL-psbF_p5	CCCCCAGTAGAGACTAGTACG	GCAATGCAGTTCATCCAACG	57.1	50.0	58.2	58.4	113 bp	1.0	68.5