Hotspot

trnF-GAA-ndhJ

LSC · 46156-46882 · 727 bp

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Pi 0.0152

MarkerSeek score 67.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 11
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AATGCCGGAAAGTTGGATCG	55.0	50.0	58.0	58.9	876-879 bp	1.0	74.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AATGCCGGAAAGTTGGATCG	50.0	50.0	58.1	58.9	840-843 bp	1.0	73.7
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	AATGCCGGAAAGTTGGATCG	55.0	50.0	57.9	58.9	864-867 bp	1.0	73.0
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	ATCGGGTGGCCTTTACGTAA	55.0	50.0	58.0	59.1	860-863 bp	1.0	72.5
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	GGAAAGTTGGATCGGGTGG	55.0	57.9	58.0	58.1	870-873 bp	1.0	72.2