Hotspot

rpoB-trnC-GCA

LSC · 25945-26965 · 1021 bp

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Pi 0.0128

MarkerSeek score 67.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1021
variable_sites: 13
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCCCGTTTTTATCCCTGAG	TTTCGTGTCTTGACTAGGCG	55.0	50.0	57.9	57.9	1121-1130 bp	1.0	80.7
rpoB-trnC-GCA_p2	CCCCCGTTTTTATCCCTGAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1097-1106 bp	1.0	80.5
rpoB-trnC-GCA_p3	CCCGTTTTTATCCCTGAGCA	TTTCGTGTCTTGACTAGGCG	50.0	50.0	57.9	57.9	1119-1128 bp	1.0	80.1
rpoB-trnC-GCA_p4	CCCGTTTTTATCCCTGAGCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1095-1104 bp	1.0	80.0
rpoB-trnC-GCA_p5	CCCCCGTTTTTATCCCTGAG	GTGTCTTGACTAGGCGACAC	55.0	55.0	57.9	58.3	1117-1126 bp	1.0	78.7