Hotspot

trnK-UUU-trnQ-UUG

LSC · 4371-6622 · 2252 bp

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Pi 0.0132

MarkerSeek score 69.00

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 2252
variable_sites: 28
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2199-2311 bp	1.0	83.0
trnK-UUU-trnQ-UUG_p2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2198-2310 bp	1.0	83.0
trnK-UUU-trnQ-UUG_p3	TACCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	2185-2297 bp	1.0	80.7
trnK-UUU-trnQ-UUG_p4	AAAGCCGAGTACTCTACCGT	AAATAAAAATGGGGCGTGGC	50.0	45.0	58.2	57.6	2256-2368 bp	1.0	80.5
trnK-UUU-trnQ-UUG_p5	AAGCCGAGTACTCTACCGTT	AAATAAAAATGGGGCGTGGC	50.0	45.0	58.2	57.6	2255-2367 bp	1.0	80.5