Hotspot

trnH-GUG-psbA

LSC · 99-524 · 426 bp

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Pi 0.0414
MarkerSeek score 79.70
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
426
variable_sites
17
pi
0.0414
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TGTCGAAGCTCCATCCACTA 55.0 50.0 58.1 58.2 467-475 bp 1.0 83.2
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TGTCGAAGCTCCATCCACTA 50.0 50.0 57.9 58.2 481-489 bp 1.0 83.0
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TGTCGAAGCTCCATCCACTA 50.0 50.0 57.9 58.2 480-488 bp 1.0 83.0
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TGTCGAAGCTCCATCCACTA 50.0 50.0 57.9 58.2 482-490 bp 1.0 83.0
trnH-GUG-psbA_p5 TGATTATGGGCGAATGACGG TGTCGAAGCTCCATCCACTA 50.0 50.0 58.1 58.2 528-536 bp 1.0 82.6