Hotspot

ycf1

SSC · 123969-129581 · 5613 bp

Back to Leptodermis

Pi 0.00391

MarkerSeek score 38.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5613
variable_sites: 71
pi: 0.00391
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTGCCCCACCGTAATTGAAA	TGGATCAATGGAAAGGGTGG	45.0	50.0	58.0	57.5	1105-1162 bp	1.0	78.1
ycf1_p2	TTGCCCCACCGTAATTGAAA	GGATCAATGGAAAGGGTGGT	45.0	50.0	58.0	57.5	1104-1161 bp	1.0	78.1
ycf1_p3	GCCCCACCGTAATTGAAAGA	TGGATCAATGGAAAGGGTGG	50.0	50.0	58.2	57.5	1103-1160 bp	1.0	76.8
ycf1_p4	AGAGGCTCCAAGATTCCCTT	TGGATCAATGGAAAGGGTGG	50.0	50.0	58.0	57.5	941-998 bp	0.888889	72.1
ycf1_p5	AGAGGCTCCAAGATTCCCTT	GGATCAATGGAAAGGGTGGT	50.0	50.0	58.0	57.5	940-997 bp	0.888889	72.1