Hotspot

ndhF

SSC · 110537-112819 · 2283 bp

Back to Leptodermis

Pi 0.00409

MarkerSeek score 44.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2283
variable_sites: 29
pi: 0.00409
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACTTCACTTTGAAAAGTCTTTCT	GAGGCGAGATCTGGTGAATC	30.4	55.0	54.6	57.9	2434-2464 bp	1.0	41.0
ndhF_p2	TCAAACTTCACTTTGAAAAGTCT	GAGGCGAGATCTGGTGAATC	30.4	55.0	54.9	57.9	2438-2468 bp	1.0	40.9
ndhF_p3	TCAAACTTCACTTTGAAAAGTCT	AGAGGCGAGATCTGGTGAAT	30.4	50.0	54.9	58.2	2439-2469 bp	1.0	40.9
ndhF_p4	TCAAACTTCACTTTGAAAAGTCT	CAAAGAGGCGAGATCTGGTG	30.4	55.0	54.9	58.4	2442-2472 bp	1.0	40.9
ndhF_p5	TCAAACTTCACTTTGAAAAGTCTT	GAGGCGAGATCTGGTGAATC	29.2	55.0	55.6	57.9	2438-2468 bp	1.0	40.9