Hotspot

ndhA

SSC · 119806-122038 · 2233 bp

Back to Leptodermis

Pi 0.00474

MarkerSeek score 54.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2233
variable_sites: 32
pi: 0.00474
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTGAGACAAGTCGTGAA	CCACCGGGATTTATCAATTTGC	40.0	45.5	55.2	58.3	2390-2480 bp	1.0	42.0
ndhA_p2	TTGTTTGAGACAAGTCGTGA	CCACCGGGATTTATCAATTTGC	40.0	45.5	55.2	58.3	2391-2481 bp	1.0	42.0
ndhA_p3	TGTTTGAGACAAGTCGTGAA	CACCGGGATTTATCAATTTGCA	40.0	40.9	55.2	57.6	2389-2479 bp	1.0	42.0
ndhA_p4	TTGTTTGAGACAAGTCGTGA	CACCGGGATTTATCAATTTGCA	40.0	40.9	55.2	57.6	2390-2480 bp	1.0	42.0
ndhA_p5	TTGTTTGAGACAAGTCGTGAA	CCACCGGGATTTATCAATTTGC	38.1	45.5	56.0	58.3	2391-2481 bp	1.0	42.0