Hotspot

infA-rps8

LSC · 79642-79813 · 172 bp

Back to Leptodermis

Pi 0.0128

MarkerSeek score 53.60

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 6
pi: 0.0128
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	ACGTCGAATCTTTCCTGAAACA	GCGGGATGGGGATTGTAATT	40.9	50.0	58.3	58.0	335-342 bp	1.0	65.3
infA-rps8_p2	ACGTCGAATCTTTCCTGAAACA	TTCCTCGAATTTTAGGCGGG	40.9	50.0	58.3	58.0	350-357 bp	1.0	64.9
infA-rps8_p3	ACGTCGAATCTTTCCTGAAACA	TCGAATTTTAGGCGGGATGG	40.9	50.0	58.3	58.0	346-353 bp	1.0	64.9
infA-rps8_p4	CGTCGAATCTTTCCTGAAACA	GCGGGATGGGGATTGTAATT	42.9	50.0	56.6	58.0	334-341 bp	1.0	64.2
infA-rps8_p5	ACGTCGAATCTTTCCTGAAAC	GCGGGATGGGGATTGTAATT	42.9	50.0	56.6	58.0	335-342 bp	1.0	64.1