Hotspot

petG-trnW-CCA

LSC · 66023-66174 · 152 bp

Back to Leptodermis

Pi 0.0235

MarkerSeek score 55.40

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 152
variable_sites: 8
pi: 0.0235
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACGTGGTGATCAGTTAGACC	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.0	58.0	177-230 bp	1.0	78.4
petG-trnW-CCA_p2	ACGTGGTGATCAGTTAGACC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.0	58.0	199-252 bp	1.0	78.3
petG-trnW-CCA_p3	ACGTGGTGATCAGTTAGACC	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.0	57.9	163-216 bp	1.0	78.2
petG-trnW-CCA_p4	GACGTGGTGATCAGTTAGACC	AGAACGTGGGTCTCCAAAAC	52.4	50.0	58.1	58.0	178-231 bp	1.0	77.7
petG-trnW-CCA_p5	GACGTGGTGATCAGTTAGACC	AGCGCTCTTAGTTCAGTTCG	52.4	50.0	58.1	58.0	200-253 bp	1.0	77.6