Hotspot

psbE-petL

LSC · 64554-65643 · 1090 bp

Back to Leptodermis

Pi 0.00647

MarkerSeek score 44.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1090
variable_sites: 19
pi: 0.00647
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGTCGTATTTTGCTCAACCCA	50.0	42.9	57.8	57.9	1198-1232 bp	1.0	71.5
psbE-petL_p2	AATAGCCAACCCGCAATGAA	AGTCGTATTTTGCTCAACCCA	45.0	42.9	58.2	57.9	1281-1315 bp	1.0	71.4
psbE-petL_p3	ATAGCCAACCCGCAATGAAA	AGTCGTATTTTGCTCAACCCA	45.0	42.9	58.2	57.9	1280-1314 bp	1.0	71.4
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AGTCGTATTTTGCTCAACCCA	55.0	42.9	58.2	57.9	1193-1227 bp	1.0	71.1
psbE-petL_p5	AACCCGCAATGAAAAGGGAA	AGTCGTATTTTGCTCAACCCA	45.0	42.9	58.3	57.9	1274-1308 bp	1.0	70.3