Hotspot

accD-psaI

LSC · 58312-59035 · 724 bp

Back to Leptodermis

Pi 0.00772

MarkerSeek score 56.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 724
variable_sites: 17
pi: 0.00772
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CGTTCCATGCTTTCCTTCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	501-886 bp	1.0	84.0
accD-psaI_p2	AGTTACTTGCGTTCCATGCT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.8	58.0	510-895 bp	1.0	83.9
accD-psaI_p3	CGTTCCATGCTTTCCTTCCT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	507-892 bp	1.0	83.4
accD-psaI_p4	AGTTACTTGCGTTCCATGCT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.8	57.9	516-901 bp	1.0	83.3
accD-psaI_p5	TTCGACCTAATTGTCCCACG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.6	58.0	556-941 bp	1.0	82.3