Pi
0.00862
MarkerSeek score
42.10
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | ATGGCTTTTGGAAAGGGGAG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 916-948 bp | 1.0 | 81.9 |
| trnT-UGU-trnL-UAA_p2 | GATGGCTTTTGGAAAGGGGA | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 917-949 bp | 1.0 | 81.8 |
| trnT-UGU-trnL-UAA_p3 | CGAAGCCATACCCAATCGAA | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1450-1483 bp | 1.0 | 81.3 |
| trnT-UGU-trnL-UAA_p4 | AAACTTGACCTGTTGACCCC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.9 | 58.0 | 1391-1424 bp | 1.0 | 81.3 |
| trnT-UGU-trnL-UAA_p5 | GCTTTTCCGGCAATACGAAC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1368-1401 bp | 1.0 | 81.0 |