Hotspot

trnK-UUU-rps16

LSC · 3887-4717 · 831 bp

Back to Leptodermis

Pi 0.00428
MarkerSeek score 58.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
831
variable_sites
14
pi
0.00428
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TGCTCAACTTACAGGAACCG 50.0 50.0 58.2 57.8 940-960 bp 1.0 80.7
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TGCTCAACTTACAGGAACCG 50.0 50.0 58.2 57.8 939-959 bp 1.0 80.7
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GCTCAACTTACAGGAACCGT 50.0 50.0 58.2 57.8 939-959 bp 1.0 80.7
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GCTCAACTTACAGGAACCGT 50.0 50.0 58.2 57.8 938-958 bp 1.0 80.7
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT ACAGGAACCGTTCAGGATCT 50.0 50.0 58.2 58.4 929-949 bp 1.0 79.2