Hotspot

ndhH

SSC · 112542-113723 · 1182 bp

Back to Leptochloa

Pi 0.000846

MarkerSeek score 59.20

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 1
pi: 0.000846
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TCCCAGTAGGAGGGTCAAAA	TGCATTTTGATGAGCCTCGT	50.0	45.0	57.6	58.2	1361 bp	1.0	75.2
ndhH_p2	TCCCAGTAGGAGGGTCAAAAT	TGCATTTTGATGAGCCTCGT	47.6	45.0	58.1	58.2	1361 bp	1.0	70.1
ndhH_p3	TCCCAGTAGGAGGGTCAAAA	TTGCATTTTGATGAGCCTCG	50.0	45.0	57.6	57.1	1362 bp	1.0	69.2
ndhH_p4	TCCCAGTAGGAGGGTCAAAA	GCATTTTGATGAGCCTCGTT	50.0	45.0	57.6	57.1	1360 bp	1.0	69.2
ndhH_p5	TCCCAGTAGGAGGGTCAAAA	TTTGCATTTTGATGAGCCTCG	50.0	42.9	57.6	57.8	1363 bp	1.0	66.9