Hotspot

rpoC2-rps2

LSC · 29586-29872 · 287 bp

Back to Leptochloa

Pi 0.00348

MarkerSeek score 43.00

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 287
variable_sites: 1
pi: 0.00348
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	ATGAGACATCAGAACCCCCA	TCATCTCTTTCAAATTGATGTTCCA	50.0	32.0	58.0	57.2	371 bp	1.0	40.2
rpoC2-rps2_p2	TGAGACATCAGAACCCCCAT	TCATCTCTTTCAAATTGATGTTCCA	50.0	32.0	58.0	57.2	370 bp	1.0	40.2
rpoC2-rps2_p3	AGAACCCCCATTTACTCCCA	TCATCTCTTTCAAATTGATGTTCCA	50.0	32.0	57.9	57.2	361 bp	1.0	40.2
rpoC2-rps2_p4	ATGAGACATCAGAACCCCCA	TGATGTTCCAATATCTTCTTGTCA	50.0	33.3	58.0	56.1	356 bp	1.0	40.2
rpoC2-rps2_p5	TGAGACATCAGAACCCCCAT	TGATGTTCCAATATCTTCTTGTCA	50.0	33.3	58.0	56.1	355 bp	1.0	40.2