Hotspot

trnC-GCA-rpoB

LSC · 18467-19521 · 1055 bp

Back to Leptochloa

Pi 0.000948

MarkerSeek score 59.30

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1055
variable_sites: 1
pi: 0.000948
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1139 bp	1.0	76.9
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1138 bp	1.0	76.9
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGCCTT	50.0	45.0	58.1	57.6	1203 bp	1.0	76.5
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	AGTTCTTCTGCCAAGCCTTG	50.0	50.0	58.1	58.4	1202 bp	1.0	76.4
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CAAGTTCTTCTGCCAAGCCT	50.0	50.0	58.1	58.4	1204 bp	1.0	76.4