Hotspot

trnD-GUC-psbM

LSC · 15884-16859 · 976 bp

Back to Leptochloa

Pi 0.00307

MarkerSeek score 60.50

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 976
variable_sites: 3
pi: 0.00307
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TCAATTGGTTAGAGCACCGC	TCATTTTGGCTGGCTGTTTT	50.0	40.0	58.6	56.7	1140-1142 bp	1.0	65.4
trnD-GUC-psbM_p2	TAGGTATGCCATACACCCCG	TCATTTTGGCTGGCTGTTTT	55.0	40.0	58.7	56.7	1173-1175 bp	1.0	64.6
trnD-GUC-psbM_p3	TCAATTGGTTAGAGCACCGC	AATCATTTTGGCTGGCTGTT	50.0	40.0	58.6	56.5	1142-1144 bp	1.0	63.6
trnD-GUC-psbM_p4	TCAATTGGTTAGAGCACCGC	ATCATTTTGGCTGGCTGTTT	50.0	40.0	58.6	56.5	1141-1143 bp	1.0	63.6
trnD-GUC-psbM_p5	TCAATTGGTTAGAGCACCGC	TCATTTTGGCTGGCTGTTTTT	50.0	38.1	58.6	57.4	1140-1142 bp	1.0	63.3