Hotspot

trnG-UCC-trnM-CAU

LSC · 13648-14638 · 991 bp

Back to Leptochloa

Pi 0.00101
MarkerSeek score 59.30
Primer available yes
Label trnG-UCC-trnM-CAU

Feature Metadata

label_name
trnG-UCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
991
variable_sites
1
pi
0.00101
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnM-CAU_p1 ACGAATCACACTTTTACCACT GGAAAAGCCCCTTATCGGAT 38.1 50.0 55.5 57.3 1096 bp 1.0 46.5
trnG-UCC-trnM-CAU_p2 AGAACGAATCACACTTTTACCAC GGAAAAGCCCCTTATCGGAT 39.1 50.0 57.3 57.3 1099 bp 1.0 45.5
trnG-UCC-trnM-CAU_p3 GAACGAATCACACTTTTACCACT GGAAAAGCCCCTTATCGGAT 39.1 50.0 57.3 57.3 1098 bp 1.0 45.5
trnG-UCC-trnM-CAU_p4 AACGAATCACACTTTTACCACT GGAAAAGCCCCTTATCGGAT 36.4 50.0 56.2 57.3 1097 bp 1.0 44.6
trnG-UCC-trnM-CAU_p5 ACGAATCACACTTTTACCACT GGAAAAGCCCCTTATCGGATT 38.1 47.6 55.5 58.1 1096 bp 1.0 43.3