Hotspot

matK-trnK-UUU

LSC · 3234-3914 · 681 bp

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Pi 0
MarkerSeek score 58.90
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
681
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 CATAATCGTGGGCAAATGCA AGGAGCGTTCGGTGTAATTC 45.0 50.0 57.1 58.0 881-882 bp 1.0 73.1
matK-trnK-UUU_p2 ACATAATCGTGGGCAAATGC AGGAGCGTTCGGTGTAATTC 45.0 50.0 57.1 58.0 882-883 bp 1.0 73.1
matK-trnK-UUU_p3 CATAATCGTGGGCAAATGCA CAGGAGCGTTCGGTGTAATT 45.0 50.0 57.1 58.3 882-883 bp 1.0 70.9
matK-trnK-UUU_p4 ACATAATCGTGGGCAAATGC CAGGAGCGTTCGGTGTAATT 45.0 50.0 57.1 58.3 883-884 bp 1.0 70.9
matK-trnK-UUU_p5 CATAATCGTGGGCAAATGCA GAGCGTTCGGTGTAATTCCA 45.0 50.0 57.1 58.3 879-880 bp 1.0 70.9