Hotspot

ndhB

Genome · 625-2995 · 2371 bp

Back to Leptochilus

Pi 0.0732

MarkerSeek score 55.20

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: Genome
length_bp: 2371
variable_sites: 3
pi: 0.0732
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.02
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	TCGCGTCACAAGAAGAGAAG	TCAATCTCAAATATTACTCCGA	50.0	31.8	57.9	52.5	2426 bp	0.5	20.0
ndhB_p2	TGCGGAGACAAACTAGTTGG	TCAATCTCAAATATTACTCCGA	50.0	31.8	57.8	52.5	2492 bp	0.5	20.0
ndhB_p3	TCGCGTCACAAGAAGAGAAG	TTCAATCTCAAATATTACTCCGA	50.0	30.4	57.9	53.3	2427 bp	0.5	20.0
ndhB_p4	AGTTCGCGTCACAAGAAGAG	TCAATCTCAAATATTACTCCGA	50.0	31.8	58.2	52.5	2429 bp	0.5	20.0
ndhB_p5	CGCGTCACAAGAAGAGAAGT	TCAATCTCAAATATTACTCCGA	50.0	31.8	58.2	52.5	2425 bp	0.5	20.0