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chlN-chlL

Genome · 131183-131262 · 80 bp

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Pi 0.1

MarkerSeek score 75.60

Primer available yes

Label chlN-chlL

Feature Metadata

label_name: chlN-chlL
feature_type: igs
region: Genome
length_bp: 80
variable_sites: 8
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN-chlL_p1	TCGCATTCGAAAGTGAGAAT	AGATGCTGCGCTTGATTTTG	40.0	45.0	55.5	57.7	146 bp	1.0	62.2
chlN-chlL_p2	TCGCATTCGAAAGTGAGAAT	CAAGATGCTGCGCTTGATTT	40.0	45.0	55.5	57.7	148 bp	1.0	62.1
chlN-chlL_p3	TCGCATTCGAAAGTGAGAAT	GCTGCGCTTGATTTTGCAAT	40.0	45.0	55.5	58.9	142 bp	1.0	57.1
chlN-chlL_p4	TCGCATTCGAAAGTGAGAAT	AAGATGCTGCGCTTGATTTT	40.0	40.0	55.5	57.0	147 bp	1.0	56.1
chlN-chlL_p5	TCGCATTCGAAAGTGAGAAT	ATGCTGCGCTTGATTTTGC	40.0	47.4	55.5	58.3	144 bp	1.0	54.4