Hotspot

rps15-ycf1

Genome · 119800-124322 · 4523 bp

Back to Leptochilus

Pi 0.0499

MarkerSeek score 69.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: Genome
length_bp: 4523
variable_sites: 199
pi: 0.0499
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CTACCGCGACTCACGTTAAA	GCCACCATATCGATCTCGTC	50.0	55.0	58.0	58.0	1134-1155 bp	1.0	85.8
rps15-ycf1_p2	GGGTTGTAGTAGGCGTTAGC	GCCACCATATCGATCTCGTC	55.0	55.0	58.1	58.0	971-992 bp	1.0	85.7
rps15-ycf1_p3	CTACCGCGACTCACGTTAAA	GGTTTGATAGGCCTCCCAAG	50.0	55.0	58.0	57.9	1207-1228 bp	1.0	85.1
rps15-ycf1_p4	CTACCGCGACTCACGTTAAA	AAACTCTCTTGCAACCCTGG	50.0	50.0	58.0	58.0	1086 bp	0.5	59.6
rps15-ycf1_p5	GGGTTGTAGTAGGCGTTAGC	AAACTCTCTTGCAACCCTGG	55.0	50.0	58.1	58.0	923 bp	0.5	59.4