Hotspot

trnP-GGG-trnL-UAG

Genome · 110101-110337 · 237 bp

Back to Leptochilus

Pi 0.0506
MarkerSeek score 73.20
Primer available yes
Label trnP-GGG-trnL-UAG

Feature Metadata

label_name
trnP-GGG-trnL-UAG
feature_type
igs
region
Genome
length_bp
237
variable_sites
12
pi
0.0506
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-GGG-trnL-UAG_p1 GTGACTTCGCTATCAGTCGG CTCTGGCACTGCTTCCTAAG 55.0 55.0 58.2 58.0 380-381 bp 1.0 81.0
trnP-GGG-trnL-UAG_p2 GTGACTTCGCTATCAGTCGG CTGGCACTGCTTCCTAAGAG 55.0 55.0 58.2 58.0 378-379 bp 1.0 81.0
trnP-GGG-trnL-UAG_p3 GTGACTTCGCTATCAGTCGG AGAGCAGCGTGTCTACCTAT 55.0 50.0 58.2 57.9 362-363 bp 1.0 80.8
trnP-GGG-trnL-UAG_p4 TGACTTCGCTATCAGTCGGA CTCTGGCACTGCTTCCTAAG 50.0 55.0 58.3 58.0 379-380 bp 1.0 80.3
trnP-GGG-trnL-UAG_p5 TGACTTCGCTATCAGTCGGA CTGGCACTGCTTCCTAAGAG 50.0 55.0 58.3 58.0 377-378 bp 1.0 80.3