Hotspot

trnW-CCA-trnP-UGG

Genome · 64584-64782 · 199 bp

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Pi 0.0553

MarkerSeek score 73.70

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: Genome
length_bp: 199
variable_sites: 11
pi: 0.0553
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	GGTAGCGTGTTTGTTTTGGG	50.0	50.0	57.9	57.9	283 bp	1.0	81.6
trnW-CCA-trnP-UGG_p2	CCTGGGGTAGACGGTAGTAG	GGTAGCGTGTTTGTTTTGGG	60.0	50.0	58.0	57.9	350 bp	1.0	81.3
trnW-CCA-trnP-UGG_p3	TAGTAGACACGCCCTGTAGG	GGTAGCGTGTTTGTTTTGGG	55.0	50.0	58.0	57.9	336 bp	1.0	81.2
trnW-CCA-trnP-UGG_p4	AATCCTGGGGTAGACGGTAG	GGTAGCGTGTTTGTTTTGGG	55.0	50.0	57.9	57.9	353 bp	1.0	80.8
trnW-CCA-trnP-UGG_p5	GTAGGATTCGAACCTACGGC	GGTAGCGTGTTTGTTTTGGG	55.0	50.0	57.9	57.9	321 bp	1.0	80.7