Hotspot

psbM-petN

Genome · 29982-31125 · 1144 bp

Back to Leptochilus

Pi 0.0502

MarkerSeek score 73.30

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 1144
variable_sites: 57
pi: 0.0502
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	CCACTTACATGCCCCAAACT	ATTGGATGTTGTTTCCGCCT	50.0	45.0	58.1	58.1	2216 bp	0.5	58.8
psbM-petN_p2	CCACTTACATGCCCCAAACT	AAGACGGAGGACTGCAAATC	50.0	50.0	58.1	57.9	2145 bp	0.5	58.6
psbM-petN_p3	CCACTTACATGCCCCAAACT	TGTTGTTTCCGCCTGGATAG	50.0	50.0	58.1	57.9	2210 bp	0.5	58.5
psbM-petN_p4	CCACTTACATGCCCCAAACT	TTGTAGTTTGGGGACGAAGC	50.0	50.0	58.1	58.1	1579 bp	0.5	58.5
psbM-petN_p5	CCACTTACATGCCCCAAACT	CATTTCATGTGCCTCGATGC	50.0	50.0	58.1	57.9	1942 bp	0.5	58.5