Hotspot

rpoB-trnD-GUC

Genome · 28492-29127 · 636 bp

Back to Leptochilus

Pi 0.0397

MarkerSeek score 74.30

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: Genome
length_bp: 636
variable_sites: 25
pi: 0.0397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	CAAGTTCTGGTAATACGGGCA	GAGTTCATCGGGATTGACGG	47.6	55.0	58.0	58.4	735-740 bp	1.0	71.8
rpoB-trnD-GUC_p2	CAAGTTCTGGTAATACGGGCA	GGAGTTCATCGGGATTGACG	47.6	55.0	58.0	58.4	736-741 bp	1.0	71.8
rpoB-trnD-GUC_p3	TGCAAGTTCTGGTAATACGGG	GAGTTCATCGGGATTGACGG	47.6	55.0	58.0	58.4	737-742 bp	1.0	71.8
rpoB-trnD-GUC_p4	TGCAAGTTCTGGTAATACGGG	GGAGTTCATCGGGATTGACG	47.6	55.0	58.0	58.4	738-743 bp	1.0	71.8
rpoB-trnD-GUC_p5	CAAGTTCTGGTAATACGGGCA	CAACTTCCGTCTTGACAGGG	47.6	55.0	58.0	58.5	703-708 bp	1.0	71.5