Hotspot

atpH-atpI

Genome · 15362-16178 · 817 bp

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Pi 0.0477
MarkerSeek score 73.80
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
Genome
length_bp
817
variable_sites
38
pi
0.0477
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 GCCTTCTACTTCTGGCTGTC TATTTCAGCCGACGAGTTGG 55.0 50.0 58.0 58.0 1108-1124 bp 1.0 84.5
atpH-atpI_p2 GCCTTCTACTTCTGGCTGTC AGGTGCCAGAAATCTGCAAA 55.0 45.0 58.0 58.0 1522-1538 bp 1.0 83.7
atpH-atpI_p3 GAGGGAGTAGCGATCTCACT AGGTGCCAGAAATCTGCAAA 55.0 45.0 58.0 58.0 2026-2031 bp 1.0 83.2
atpH-atpI_p4 GAGGGGTAAAGGTGCAACAA AGGTGCCAGAAATCTGCAAA 50.0 45.0 58.0 58.0 1685 bp 0.5 59.9
atpH-atpI_p5 GAGGGGTAAAGGTGCAACAA TATTTCAGCCGACGAGTTGG 50.0 50.0 58.0 58.0 1271 bp 0.5 59.9