Hotspot

trnG-UCC-trnR-UCU

Genome · 11526-11692 · 167 bp

Back to Leptochilus

Pi 0.0479

MarkerSeek score 73.10

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: Genome
length_bp: 167
variable_sites: 8
pi: 0.0479
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	ACGTTCGGTTCTGGATTAGC	AACACGGAATTACGCCCAAT	50.0	45.0	58.0	58.2	351 bp	1.0	80.4
trnG-UCC-trnR-UCU_p2	ACGTTCGGTTCTGGATTAGC	ACGGAATTACGCCCAATAGG	50.0	50.0	58.0	57.8	348 bp	1.0	79.8
trnG-UCC-trnR-UCU_p3	TACTGATGCGGGTTCGATTC	AACACGGAATTACGCCCAAT	50.0	45.0	57.8	58.2	281 bp	1.0	79.2
trnG-UCC-trnR-UCU_p4	CGCCGTCGACTATAACCTTT	AACACGGAATTACGCCCAAT	50.0	45.0	57.8	58.2	313 bp	1.0	79.1
trnG-UCC-trnR-UCU_p5	CTACTGATGCGGGTTCGATT	AACACGGAATTACGCCCAAT	50.0	45.0	57.8	58.2	282 bp	1.0	79.1