Hotspot

rps18

LSC · 68071-68558 · 488 bp

Back to Leptaspis

Pi 0.00205

MarkerSeek score 45.00

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 488
variable_sites: 1
pi: 0.00205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	AGAATCGCCACAATACACCC	CGTTTGTGGATCACTCGGAT	50.0	50.0	58.0	58.0	1216-1238 bp	1.0	81.0
rps18_p2	AAGGAATCGCCAGGGATTTC	CGTTTGTGGATCACTCGGAT	50.0	50.0	57.9	58.0	1253-1275 bp	1.0	80.8
rps18_p3	AGAATCGCCACAATACACCC	CGTGGTTGGGAAGGTCATAG	50.0	55.0	58.0	58.0	1532-1554 bp	1.0	80.6
rps18_p4	AAAGGAGGTTCATGGCCAAG	CGTTTGTGGATCACTCGGAT	50.0	50.0	58.1	58.0	1336-1358 bp	1.0	80.6
rps18_p5	AAGGAATCGCCAGGGATTTC	CGTGGTTGGGAAGGTCATAG	50.0	55.0	57.9	58.0	1569-1591 bp	1.0	80.5