Hotspot

rps15-trnN(GUU)

IRa · 119475-121006 · 1532 bp

Back to Leptaspis

Pi 0.00271

MarkerSeek score 63.80

Primer available yes

Label rps15-trnN(GUU)

Feature Metadata

label_name: rps15-trnN(GUU)
feature_type: igs
region: IRa
length_bp: 1532
variable_sites: 4
pi: 0.00271
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN(GUU)_p1	CAGAACCCCTGTTTTCTTCT	ACGACCAGTCAGTTAACAGC	45.0	50.0	55.2	57.8	1598-1654 bp	1.0	59.1
rps15-trnN(GUU)_p2	CAGAACCCCTGTTTTCTTCT	AGGATTCGAACCTACGACCA	45.0	50.0	55.2	58.2	1611-1667 bp	1.0	59.0
rps15-trnN(GUU)_p3	CAGAACCCCTGTTTTCTTCT	GGATTCGAACCTACGACCAG	45.0	55.0	55.2	57.8	1610-1666 bp	1.0	58.6
rps15-trnN(GUU)_p4	ACTTGAAATTCAACAGAACCCC	ACGACCAGTCAGTTAACAGC	40.9	50.0	57.1	57.8	1611-1667 bp	1.0	58.2
rps15-trnN(GUU)_p5	ACTTGAAATTCAACAGAACCCC	AGGATTCGAACCTACGACCA	40.9	50.0	57.1	58.2	1624-1680 bp	1.0	58.2