Hotspot

rpl32-trnL(UAG)

SSC · 109470-110169 · 700 bp

Back to Leptaspis

Pi 0.01

MarkerSeek score 64.50

Primer available yes

Label rpl32-trnL(UAG)

Feature Metadata

label_name: rpl32-trnL(UAG)
feature_type: igs
region: SSC
length_bp: 700
variable_sites: 7
pi: 0.01
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL(UAG)_p1	TCAAGATCATTTCCCAGCGG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	811-834 bp	1.0	79.3
rpl32-trnL(UAG)_p2	TCAAGATCATTTCCCAGCGG	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	804-827 bp	1.0	78.9
rpl32-trnL(UAG)_p3	TCAAGATCATTTCCCAGCGG	GCTTGAGCACTGCTTCCTAA	50.0	50.0	58.0	58.2	825-848 bp	1.0	78.8
rpl32-trnL(UAG)_p4	TCAAGATCATTTCCCAGCGG	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.0	57.8	810-833 bp	1.0	78.8
rpl32-trnL(UAG)_p5	TCAAGATCATTTCCCAGCGG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	809-832 bp	1.0	78.8