Hotspot

ndhF-rpl32

SSC · 108383-109289 · 907 bp

Back to Leptaspis

Pi 0.015

MarkerSeek score 62.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 907
variable_sites: 13
pi: 0.015
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGGAGAAAAGGT	TCCAAAGATTTTTACGAATACGCT	50.0	33.3	57.0	57.3	977-1020 bp	1.0	44.5
ndhF-rpl32_p2	CTGGAAGTGGGAGAAAAGGT	TCCAAAGATTTTTACGAATACGC	50.0	34.8	57.0	55.9	977-1020 bp	1.0	44.5
ndhF-rpl32_p3	CTGGAAGTGGGAGAAAAGGT	CCAAAGATTTTTACGAATACGCT	50.0	34.8	57.0	55.9	976-1019 bp	1.0	44.5
ndhF-rpl32_p4	CTGGAAGTGGGAGAAAAGGT	TCCAAAGATTTTTACGAATACGCTT	50.0	32.0	57.0	57.9	977-1020 bp	1.0	44.5
ndhF-rpl32_p5	CTGGAAGTGGGAGAAAAGGT	TTCCAAAGATTTTTACGAATACGCT	50.0	32.0	57.0	57.9	978-1021 bp	1.0	44.5