Hotspot

rpl33-rps18

LSC · 67842-68070 · 229 bp

Back to Leptaspis

Pi 0.0407

MarkerSeek score 79.10

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 229
variable_sites: 9
pi: 0.0407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AGAATCGCCACAATACACCC	TGGATTTACGAAAGGGTTGCT	50.0	42.9	58.0	57.9	376-395 bp	1.0	74.0
rpl33-rps18_p2	CCACAATACACCCCGACAAT	TGGATTTACGAAAGGGTTGCT	50.0	42.9	57.9	57.9	369-388 bp	1.0	73.4
rpl33-rps18_p3	CGCAAGCATACGACTCATGA	TGGATTTACGAAAGGGTTGCT	50.0	42.9	58.4	57.9	320-339 bp	1.0	71.4
rpl33-rps18_p4	TCGCAAGCATACGACTCATG	TGGATTTACGAAAGGGTTGCT	50.0	42.9	58.4	57.9	321-340 bp	1.0	71.4
rpl33-rps18_p5	TGTCGTTATTGTCGCAAGCA	TGGATTTACGAAAGGGTTGCT	45.0	42.9	58.5	57.9	332-351 bp	1.0	70.8