Hotspot

ycf3-trnS(GAA)

LSC · 45610-46257 · 648 bp

Back to Leptaspis

Pi 0.00951

MarkerSeek score 63.90

Primer available yes

Label ycf3-trnS(GAA)

Feature Metadata

label_name: ycf3-trnS(GAA)
feature_type: igs
region: LSC
length_bp: 648
variable_sites: 6
pi: 0.00951
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS(GAA)_p1	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	831-836 bp	1.0	80.5
ycf3-trnS(GAA)_p2	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.1	57.8	781-786 bp	1.0	80.3
ycf3-trnS(GAA)_p3	TTCTCCTGAGGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	832-837 bp	1.0	78.7
ycf3-trnS(GAA)_p4	TTTCTCCTGAGGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	833-838 bp	1.0	78.7
ycf3-trnS(GAA)_p5	TTCTCCTGAGGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.3	57.8	782-787 bp	1.0	78.5