Hotspot

atpH-atpF

LSC · 33914-34444 · 531 bp

Back to Leptaspis

Pi 0.021

MarkerSeek score 70.40

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 531
variable_sites: 11
pi: 0.021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	TTATGGACTGGTTGTGGCAC	ATTAAGCCCGAAACTCCCAG	50.0	50.0	58.1	57.6	646-652 bp	1.0	78.0
atpH-atpF_p2	TGTGGCACTAGCGCTTTTAT	ATTAAGCCCGAAACTCCCAG	45.0	50.0	57.9	57.6	634-640 bp	1.0	77.9
atpH-atpF_p3	TTGTGGCACTAGCGCTTTTA	ATTAAGCCCGAAACTCCCAG	45.0	50.0	58.1	57.6	635-641 bp	1.0	77.9
atpH-atpF_p4	TTATGGACTGGTTGTGGCAC	GATGGCCAGTGACCCAAAAA	50.0	50.0	58.1	58.7	624-630 bp	1.0	76.1
atpH-atpF_p5	TGTGGCACTAGCGCTTTTAT	GATGGCCAGTGACCCAAAAA	45.0	50.0	57.9	58.7	612-618 bp	1.0	76.0