Hotspot

trnT(GGU)-trnE(UUC)

LSC · 16373-16881 · 509 bp

Back to Leptaspis

Pi 0.02
MarkerSeek score 69.70
Primer available yes
Label trnT(GGU)-trnE(UUC)

Feature Metadata

label_name
trnT(GGU)-trnE(UUC)
feature_type
igs
region
LSC
length_bp
509
variable_sites
10
pi
0.02
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT(GGU)-trnE(UUC)_p1 GGCCCTTTTAACTCAGTGGT GCTGCCTCCTTGAAAGAGAG 50.0 55.0 57.7 58.3 625-630 bp 1.0 78.0
trnT(GGU)-trnE(UUC)_p2 GGCCCTTTTAACTCAGTGGT TGTCCTGAACCACTAGACGA 50.0 50.0 57.7 57.7 604-609 bp 1.0 78.0
trnT(GGU)-trnE(UUC)_p3 AGGCCCTTTTAACTCAGTGG GCTGCCTCCTTGAAAGAGAG 50.0 55.0 57.4 58.3 626-631 bp 1.0 75.6
trnT(GGU)-trnE(UUC)_p4 AGGCCCTTTTAACTCAGTGG TGTCCTGAACCACTAGACGA 50.0 50.0 57.4 57.7 605-610 bp 1.0 75.6
trnT(GGU)-trnE(UUC)_p5 GGCCCTTTTAACTCAGTGGT AACCACTAGACGATAGGGGC 50.0 55.0 57.7 58.6 597-602 bp 1.0 75.5