Hotspot

trnG(UCC)-trnT(GGU)

LSC · 13224-16300 · 3077 bp

Back to Leptaspis

Pi 0.0124

MarkerSeek score 56.50

Primer available yes

Label trnG(UCC)-trnT(GGU)

Feature Metadata

label_name: trnG(UCC)-trnT(GGU)
feature_type: igs
region: LSC
length_bp: 3077
variable_sites: 38
pi: 0.0124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG(UCC)-trnT(GGU)_p1	TTAAGGGAATTTCGGCGGAG	TTTGGCAGTTCGTTCGATCT	50.0	45.0	58.0	57.8	699 bp	0.5	58.4
trnG(UCC)-trnT(GGU)_p2	ATTTCGGCGGAGGATTCTTC	TTTGGCAGTTCGTTCGATCT	50.0	45.0	58.0	57.8	691 bp	0.5	58.3
trnG(UCC)-trnT(GGU)_p3	GAATTTCGGCGGAGGATTCT	TTTGGCAGTTCGTTCGATCT	50.0	45.0	58.0	57.8	693 bp	0.5	58.3
trnG(UCC)-trnT(GGU)_p4	CCCGCCTTTGTCCAATCTAA	TTTGGCAGTTCGTTCGATCT	50.0	45.0	57.9	57.8	753 bp	0.5	57.7
trnG(UCC)-trnT(GGU)_p5	TTAAGGGAATTTCGGCGGAG	CCAATTTGGCAGTTCGTTCG	50.0	50.0	58.0	58.3	703 bp	0.5	57.2