Hotspot

psbZ-trnG(UCC)

LSC · 12843-13151 · 309 bp

Back to Leptaspis

Pi 0.0256

MarkerSeek score 57.70

Primer available yes

Label psbZ-trnG(UCC)

Feature Metadata

label_name: psbZ-trnG(UCC)
feature_type: igs
region: LSC
length_bp: 309
variable_sites: 5
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.63
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG(UCC)_p1	TTGCTTCTCCTGATGGTTGG	TCGAACCCGTATCTTCTCCT	50.0	50.0	57.8	57.6	360-474 bp	1.0	79.9
psbZ-trnG(UCC)_p2	GCTTCTCCTGATGGTTGGTC	TCGAACCCGTATCTTCTCCT	55.0	50.0	58.3	57.6	358-472 bp	1.0	79.5
psbZ-trnG(UCC)_p3	TTGCTTCTCCTGATGGTTGG	CCGTATCTTCTCCTTGGCAA	50.0	50.0	57.8	57.3	354-468 bp	1.0	77.8
psbZ-trnG(UCC)_p4	GCTTCTCCTGATGGTTGGTC	CCGTATCTTCTCCTTGGCAA	55.0	50.0	58.3	57.3	352-466 bp	1.0	77.4
psbZ-trnG(UCC)_p5	TTGCTTCTCCTGATGGTTGG	AAATTAAGCTGGCGAACGAC	50.0	45.0	57.8	57.1	385-499 bp	1.0	76.1