Hotspot

trnK(UUU)-rps16

LSC · 3995-4595 · 601 bp

Back to Leptaspis

Pi 0.0119

MarkerSeek score 67.10

Primer available yes

Label trnK(UUU)-rps16

Feature Metadata

label_name: trnK(UUU)-rps16
feature_type: igs
region: LSC
length_bp: 601
variable_sites: 7
pi: 0.0119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK(UUU)-rps16_p1	CGTTGAGTTAGCAACCCAGA	GGTGCTCAACCTACAAGAACT	50.0	47.6	57.8	57.9	695-707 bp	1.0	71.8
trnK(UUU)-rps16_p2	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAAGAACT	50.0	47.6	58.2	57.9	712-724 bp	1.0	71.8
trnK(UUU)-rps16_p3	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAAGAACT	50.0	47.6	58.2	57.9	711-723 bp	1.0	71.8
trnK(UUU)-rps16_p4	TACCGTTGAGTTAGCAACCC	GGTGCTCAACCTACAAGAACT	50.0	47.6	57.5	57.9	698-710 bp	1.0	69.5
trnK(UUU)-rps16_p5	AGTCGCACTTAAAAGCCGAG	GGTGCTCAACCTACAAGAACT	50.0	47.6	58.6	57.9	723-735 bp	1.0	68.5