Hotspot

EI144_pgp62

LSC · 5014-6308 · 1295 bp

Back to Lepocinclis

Pi 0.221

MarkerSeek score 84.20

Primer available yes

Label EI144_pgp62

Feature Metadata

label_name: EI144_pgp62
feature_type: gene
region: LSC
length_bp: 1295
variable_sites: 474
pi: 0.221
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
EI144_pgp62_p1	TCGGTCCATATCCGGTGTAA	ACGCCAAAACTAGGAGCAAA	50.0	45.0	57.9	58.0	2544-2630 bp	0.75	79.5
EI144_pgp62_p2	TTCGGTCCATATCCGGTGTA	ACGCCAAAACTAGGAGCAAA	50.0	45.0	57.9	58.0	2545-2631 bp	0.75	79.5
EI144_pgp62_p3	GAGAGGACCGAAAAGGACAC	ACGCCAAAACTAGGAGCAAA	55.0	45.0	57.9	58.0	2490-2576 bp	0.75	79.5
EI144_pgp62_p4	TCGGTCCATATCCGGTGTAA	CAAAAAGAGCAGCGCCTAAC	50.0	50.0	57.9	58.0	2688 bp	0.25	49.2
EI144_pgp62_p5	TTCGGTCCATATCCGGTGTA	CAAAAAGAGCAGCGCCTAAC	50.0	50.0	57.9	58.0	2689 bp	0.25	49.2